Biology template strand

In eukaryotes splicing takes place in order to remove the non-coding regions of the DNA to gives the final mRNA strand. Then further the mRNA strand leaves the nucleus through the nuclear pore and is then translated by the ribosome, which is one of the cell organelles to form a sequence of amino acids Ans forms a necessary protein. Template strand consists of an anticodon.

It contains the same nucleotide sequences as such of the tRNA. Where the codon and template strand are the two different strands of the double strand DNA. The template strand acts as a base for transcribing the mRNA and the others determines the appropriate base sequence for the mRNA.

It has a nucleotide base sequence which is complementary to both the coding strands and also to the mRNA. During genetic coding the template strands have the anticodons. During the formation of hydrogen bonds, the bonds formed in the template strands are temporary and whereas the newly synthesised mRNA occurs at the time of transcription. As said before template strand is one of the DNA strands whose sequence of bases helps in building up the mRNA through the complementary base sequencing. Template strand contains the complementary nucleotide sequences which are further transcribes into the m-RNA.

After the process of transcription, the mRNA is converted into the mature mRNA, which undergoes specific post-transcriptional modifications. The template strand also contains the anticodons which carries the triplet codes or the triplet nucleotide sequences complementary to anticodon sequence of a t-RNA. The anticodon thus helps in attaching the specific amino acid to the t-RNA and forms proteins or a polypeptide chain through the assistance of the r-RNA. An enzyme RNA polymerase reads the template strand to synthesis the RNA transcript by recognising the specific sequences.

Hence, RNA polymerase is considered as the one which decides the initiation of the transcription and also in the termination of the translation process.

Template and coding strands are the terms generally used to describe the strands which are present in the DNA. During the process of transcription, one of the two strands in the double stranded DNA serves as a template strand. Where as the other strand which is present in the DNA, other than the template strand is known as coding strand. Template strand is responsible for the sequencing amino acid for synthezing the polypeptide chain. The main difference to be considered between the coding and template strand is that the template strand serves as the template for the transcription where the coding strand contains the exact and the same sequence of the nucleotides in mRNA, expect the nucleotide thymine.

The template strand is the strand which serves as the template for the mRNA synthesis during transcription. That means, the template strand is the DNA strand in the double-stranded DNA which is responsible for the amino acid sequence of the synthesized polynucleotide chain.

The template strand is also called the antisense strand or the positive strand. The template strand consists of a sequence of anti-codons which are the nucleotide triplets found in the tRNA s individually. The anti-codon is complementary to codons in the non-template or the coding strand.

The synthesizing mRNA is temporally attached to the template strand by forming hydrogen bonds with the complementary nucleotides in the template strand. RNA polymerase adds uracil as the complementary nucleotide to the mRNA strand for adenine in the template strand instead of thymine. The template strand in the transcription is shown in figure 1. The DNA strand which serves as the non-template strand during transcription is referred to as the coding strand.

Hence, coding strand is incapable of serving as the template during transcription. The coding strand contains codons, which are the nucleotide triplets which specify a unique amino acid in the polypeptide chain. These codons collectively make the genetic code, which is a universal feature in almost all the living forms on the earth.

Coding strand during the transcription is shown in figure 2. The difference between template and coding strand is mainly due to the following properties: directional polarity and function. Both template and coding strand are the two distinct strand of the double-stranded DNA, in which the former works as a base to transcribe mRNA, and the latter determines the correct base sequence of the mRNA.

In this session, we will primarily focus on the key differences between the template and coding strand along with the comparison chart.

In addition to this, you will get to know the definition and examples of the two. The template strand is one of the DNA strands whose base sequence helps in building mRNA through complementary base sequencing. It contains complementary nucleotide sequences to the transcribed mRNA. After transcription, the mRNA before converted into mature mRNA, it undergoes certain post-transcriptional modifications. The anticoding helps in the attachment of the specific amino acid to the t-RNA to form protein or a peptide chain via the assistance of rRNA.